'Tu-whoo' definitions:

Definition of 'Tu-whoo'

From: GCIDE

Tu-whit \Tu-whit"\, Tu-whoo \Tu-whoo"\, n. & interj. Words imitative of the notes of the owl. [1913 Webster]
Thy tu-whits are lulled, I wot, Thy tu-whoos of yesternight. --Tennyson. [1913 Webster]